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. 2016 May 4;11(5):e0152730. doi: 10.1371/journal.pone.0152730

Table 2. Primer sequences and amplicon characteristics of candidate reference genes for qRT-PCR studies in brown marmorated stink bug.

Gene symbol Primer sequences Amplicon Length (bp) Product Tm (°C) Amplification efficiency E (%) Correlation coefficient (R2)
RPS26 CCTACCAAAGCCTTCTGAATATACCGTAATTGCCATAAGAG 79 79.0 94.98 0.92
EF1A GCTGATTGTGCTGTGTTAACGAGTCTGTCCATTCTT 78 79.0 104.47 0.99
FAU GGAACTGTGAGGTCAAGATTAGCAGCATCAGGAACT 80 76.5 85.58 0.98
UBE4A CGACCATCCTTAGAGACAGATTACTGCCATGCTCAA 183 78.5 98.39 0.96
ARL2 AGTTCTCGTGACTAATCGCTTGAATACCTGTCCAGTA 127 76.5 86.31 0.92
ARP8 CCAACAATCAGCGAAGGTATGAGCAACTACTTCAGGAATAACTCT 80 76.5 103.42 0.95
GUS ATCGTATCAGCACCGTATTTGAAGCAGAAGCAGAAC 76 79.0 112.60 0.93
TBP GTATATTGGTGGAAATGAAGATAAGTATCCAAGCAT 97 75.0 95.98 0.93
TIF6 CACGCATTGTTGTAGTAATGCTGGTATGGTAGTGAAT 130 78.5 82.66 0.92
RPL9 CGGAGACATAAAGACCATAAGGACGAACTTATTATTGAG 127 78.5 82.57 0.99