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. 2016 May 4;11(5):e0153780. doi: 10.1371/journal.pone.0153780

Fig 3. LMOD1 and MEF2C are validated as a direct target of miR-214.

Fig 3

(A) miR-214 binding sites in the 3’-UTR of LMOD1 and MEF2C transcripts. Seed and target sequences are in blue, and base-pairing between miR-214 and target site marked by vertical lines. Mutations in the reporter plasmid construct are indicated in red letters. The double mutant (not shown) carries mutations to both sites in the 3’UTR construct vs. the single site mutants shown in 3A. (B-E) For luciferase assays, HEK293 cells (B, D) or hPASMCs (C, E) were transfected with miR-214 mimic or non-targeting mimic control and wildtype vs. mutant constructs for LMOD1 (B, C) or MEF2C (D, E). Relative luciferase activities were determined 48 hrs post transfection. (n = 3–5), $, p<0.05 WT scrmb vs. mimic; *, p<0.05 WT mimic vs. mutants mimic.