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. 2016 May 4;11(5):e0154874. doi: 10.1371/journal.pone.0154874

Fig 14. MAP3K19 antagonist inhibited TGF-β1-induced protein and acted additively with therapeutic doses of pirfenidone.

Fig 14

A549 cells were treated with 30 μM, 3 μM, 300 nM or 30 nM of Compound A or 5000 μM, 1700 μM, 560 μM or 190 μM of pirfenidone alone or in combination, and TGF-β1 (1 ng/ml) for 24 hrs. Cell lysates were isolated and PAI-1 (Serpin-1) protein levels were determined by ELISA. Results shown were the percentage of PAI-1 protein compared to levels from cells treated only with TGF-β1. Results are representative of two independent experiments.