Fig. 6.

NMR results implicating LID domain effects due to entropy promoting mutations. (a) ¹H–¹⁵N HSQC spectra of wild-type (black) and mutant V142G AK(e) at 33°C. Labels indicate those residues that were assignable from published values [75] and also exhibited measurable intensity in the wild-type spectrum but no measurable intensity in V142G. (b) The same residues labeled in Fig. 6a are shown as yellow sticks, projected onto the “open” conformation of AK(e) (PDB ID 4AKE)