Fig. 7.

CPMG results for apo V142G are consistent with all-or-none local unfolding of the LID domain. (a) Individual fits of the data, i.e., data points for each residue are fitted to (6) to obtain many sets of fitted parameters. These fitted parameters are individually listed in Table 2. (b) Global fit of all data points from all residues to a hypothetical two state transition between folded AK(e) and random coil chemical shifts. This one set of fitted parameters is given in Table 2 in the row labeled “Global”. Relaxation dispersion data was collected for apo V142G AK(e) at 19°C in 50 mM MOPS, 50 mM NaCl, 20 mM MgCl₂, 5% glycerol, 8 mM DTT, 8 mM TCEP, pH 7.85. Data were collected at both 800 and 600 MHz field strength; 800 MHz results are displayed