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. Author manuscript; available in PMC: 2016 Sep 23.
Published in final edited form as: Nature. 2016 Mar 23;531(7596):604–609. doi: 10.1038/nature17394

Extended Data Figure 1. Cryo-EM of the TFIID-IIA-SCP complex.

Extended Data Figure 1

a, Representative micrograph of frozen-hydrated TFIID-IIA-SCP complexes. Examples of particle picks are indicated by the green circles. 203,163 such picks were made from 1,253 total micrographs. b, Initial classification and refinement scheme for the TFIID-IIA-SCP structure (see Methods). c, Idealized dose-dependent B-factor plot based on cryo-EM data collected on microtubules under similar imaging conditions. This plot was used for the particle polishing step in b. d, e, Fourier shell correlation plot (d) and local resolution estimation (e) for the final reconstruction shown in b.