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. Author manuscript; available in PMC: 2016 Sep 23.
Published in final edited form as: Nature. 2016 Mar 23;531(7596):604–609. doi: 10.1038/nature17394

Extended Data Figure 2. Focused classification and refinement of the promoter-bound BC-core and lobe C of TFIID.

Extended Data Figure 2

a, b, Scheme for focused classification and refinement of the BC-core region (a) or lobe C region of the TFIID-IIA-SCP structure (b) (see Methods). c, d, Fourier shell correlation plots (c) and local resolution estimations (d) of the BC-core and lobe C maps, corresponding to the final structures shown in a and b, respectively. e, Two-dimensional projections of the refined maps for the full TFIID-IIA-SCP structure (left), locally-refined BC-core map (middle), and locally-refined lobe C map (right). The maps used to calculate the projections are the same as the final structures in a, b, and Extended Data Fig. 1b, except that all have been low-pass filtered to 10 Å before calculating projections. f, 3D-classification of 56,457 particles into two classes (solid blue and transparent green), following focused alignment to the lobe C region of the structure. The resulting classes have been superposed through their lobe C densities in order to illustrate the flexibility of lobe B and the upstream region of promoter DNA relative to lobe C and the downstream promoter region. The magnitude of motion within lobe A1 (20 Å) is indicated.