Fig 2. Whole-genome sequencing of L638^R mutants.

Heat map summary of all non-synonymous mutations identified by Illumina-based whole-genome sequencing (100X genome coverage) of L638^R mutants in MRSE CLB26329 (A) or MRSA COL (B). Red, non-synonymous mutation; grey, no change versus parental genome sequence; yellow, non-synonymous mutations in genes other than mnaA. Genome position, base pair change, and resulting amino acid residue substitution are highlighted. Note: with only one exception (Δcap5P mnaA _Sa ^D281Y), no additional non-synonymous mutations besides the indicated mnaA mutation were identified in each of the drug resistant strains examined.