Table 1. Antibodies and their respective dilutions in specific assays.
| Assay | |||||
|---|---|---|---|---|---|
| Primary antibodies | Species | WB | IP | IF | Source |
| p53 | Mouse | 1:1000 | 1:100 | - | Santa Cruz, sc-126 |
| Plakoglobin | Mouse | 1:1000 | 1:100 | 1:100 | Translab, 610254 |
| E-cadherin | Mouse | 1:1000 | - | 1:100 | Translab, 610404 |
| N-cadherin | Mouse | 1:1000 | - | - | Santa Cruz, sc-59987 |
| Cytokeratin (pan-keratin) | Mouse | 1:1000 | - | - | Sigma, C-2931 |
| Vimentin | Mouse | 1:1000 | - | - | Sigma, V-6630 |
| β-actin | Mouse | 1:1000 | - | - | Santa Cruz, sc-47778 |
| Secondary antibodies | |||||
| Anti-mouse IgG, light chain | Goat | 1:15000 | - | - | Jackson Immuno Research, 115-625-174 |
| Anti-rabbit IgG, light chain | Goat | 1:15000 | - | - | Jackson Immuno Research, 211-652-171 |
| Alexa fluor 488 | Mouse | - | - | 1:2000 | Molecular Probes, A11029 |
| Alexa fluor 546 | Rabbit | - | - | 1:3000 | Molecular Probes, A11035 |
| Rhodamine | Rabbit | - | - | 1:400 | Boehringer Mannheim, 605107 |
| Rhodamine | Rat | - | - | 1:400 | Sigma, T4280 |