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. 2016 May 4;11(5):e0153979. doi: 10.1371/journal.pone.0153979

Fig 4.

Fig 4

(A-D) Electron micrographs of asymmetric synapses from dissociated hippocampal cultures labeled for Shank3 using ab2. Scale bars = 0.1 μm (A and B share the same scale bar, C and D share the same scale bar). Labeling intensity increased upon 2 min treatment with either high K+ (B vs. A) or NMDA (D vs. C). (E, F) Bar graphs represent combined data (mean ± SEM) from S1 and S2 Tables. (G-J) Histograms from a typical experiment depicting frequency distribution of gold particles as a function of distance from the postsynaptic membrane. Median distance of label from postsynaptic membrane increased after high K+ (H vs. G) or NMDA treatment (J vs. I), indicating preferential recruitment of Shank3 molecules to the distal layer of the PSD.