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. 2016 Apr 6;6(5):372–385. doi: 10.1002/2211-5463.12052

Figure 1.

Figure 1

C‐terminal ATPase domain of Pyrococcus furiosus RadA is monomeric (A) Domain structure of a single protomer of full‐length RadA from Pyrococcus furiosus (PDB: 1PZN) showing the different structural units in different colours (B) SDS/PAGE gel of E. coli expression tests for both full‐length proteins and C‐terminal domains of P. furiosus RadA and human RAD51; total lysate (T), soluble fraction (S) and uninduced control (UI). (C) Multi‐angle laser light scattering chromatogram showing protein elution monitored by the change in refractive index (blue) and molecular weight calculated from light scattering data (red). (D) Nondissociative mass spectrum showing ions with mass/charge ratios consistent with a monomeric C‐terminal RadA.