Table 1.

Results of the fluorescence‐based thermal shift and isothermal titration calorimetric analyses of nucleotide binding to RAD51–BRC4 and monomeric HumRadA2. The difference in the melting temperatures between the apo protein and the ligand‐stabilised proteins is denoted as ΔT _m

	RAD51–BRC4		HumRadA2
	ΔT m/°C	K D/μm	ΔT m/°C	K D/μm
ATP	9.0	1.0	10.0	1.0
ADP	7.0	1.0	7.0	0.54
AMPPNP	4.0	12.0	4.5	20.0
AMP	0.0	n/a	0.0	n/a
GTP	9.5	5.0	8.0	0.64
GDP	8.0	2.5	7.5	0.21