Table 1.

Sequences of primers for variants of tPA‐GFP plasmid vector

Thr‐to‐Asn at amino acid position 103 (T103N)

Forward 5′‐CAG CTA CAG GGG CAA CTG GAG CAC AGC GG‐3′ Reverse 5′‐CCG CTG TGC TCC AGT TGC CCC TGT AGC TG‐3′

Asn‐to‐Gln at amino acid position 117 (N117Q)

Forward 5′‐GTG CAC CAA CTG GCA GAG CAG CGC GTT GG‐3′ Reverse 5′‐CCA ACG CGC TGC TCT GCC AGT TGG TGC AC‐3′

Lys, His‐to‐Ala, Ala at amino acid position 296–297 (K296A, H297A)

Forward 5′‐GCT GCC ATC TTT GCC GCG GCG AGG AGG TCG CCC GG‐3′ Reverse 5′‐CCG GGC GAC CTC CTC GCC GCG GCA AAG ATG GCA GC‐3′

Arg. Arg‐to‐Ala, Ala at amino acid position 298–299 (R298A, R299A)

Forward 5′‐CCA TCT TTG CCG CGG CGG CGG CGT CGC CCG GAG AGC G‐3′ Reverse 5′‐CGC TCT CCG GGC GAC GCC GCC GCC GCG GCA AAG ATG G‐3′