Figure 4. T regulatory cell function is impaired in Stat5b-deficient mice.

(A) Contour plots show percentages of IL-2Rα⁺ cells within the FOXP3⁺ Treg compartment in spleens of 8 week-old mice. (B) Box plots show log2 fold changes in the ratio of IL-2Rα⁺/IL-2Rα^- Treg (WT=0; not shown). LN (top) and spleen (bottom) data are compiled from 5 experiments (4–6 mice/group) and genotypes ordered as in Figure 1D. (C) IL-2Rα⁺ Treg cells from WT and Stat5a- or Stat5b-deficient mice were used for in vitro suppression assays. Histograms show CFSE dilution of responder T cells. (D) Line graph shows percent suppression across a range of responder:Treg ratios. Baseline is set according to WT controls at a 1:1 ratio. Data are compiled from 3 experiments. (E) Line graph shows the percent Treg cells that remained IL-2Rα⁺ during in vitro suppression. (F) IL-2Rα⁺ Treg cells were cultured with IL-2 for 72 hr. Contour plots show the percentage of FOXP3⁺ Treg cells expressing TBX21 (top) or IL-2(bottom). (G) Box plots show log2 fold changes for TBX21⁺, FOXP3⁺ and IL-2⁺ cells relative to wild type controls (WT=0; not shown). Data are compiled from 3 experiments. Dotted red lines indicate two-fold changes.

DOI: http://dx.doi.org/10.7554/eLife.08384.007

Figure 4—figure supplement 1. Impact of Stat5 allele depletion on Treg cells.

Bar graphs show total numbers of Treg cells in spleens and LNs of 8 week old mice (4–6 mice per genotype). Number of Stat5a, Stat5b and total Stat5 alleles (i.e. genotype) is explained in the key below each graph. Error bars indicate standard deviation.

DOI: http://dx.doi.org/10.7554/eLife.08384.008