Fig. 2.
Inter-adipose septum (IAS). a Multiphoton excitation fluorescences (MPEF) demonstrating highly organised, dense structure typical of IAS in the examined gluteal bursa tissue. b SHG signal captured from the same area as (a) demonstrates that the bulk of the IAS is composed of fibrillar collagen (red and yellow). c Merged MPEF/SHG image. Note the distinct presence of longitudinally oriented, non-collagenous fibrillar structures in the IAS. d The collagenrich fibrous band in the middle of the picture is the inter-adipose septum, and the latticework of collagen fibres can also be seen in and amongst the adipocytes, H&E staining, ×100 original magnification (a–c scale bar 25.4 μm. d scale bar 100 μm)
