Table 1.
Grade 0 | Grade 1 | Grade 2 | Grade 3 | |
---|---|---|---|---|
Cell morphology Four fields of view, 200× |
Inconspicuous elongated spindle shaped nuclei with no obvious cytoplasm at light microscopy | Increased roundness: nucleus becomes more ovoid to round in shape without conspicuous cytoplasm | Increased roundness and size; the nucleus is round, slightly enlarged and a small amount of cytoplasm is visible | Nucleus is round, large with abundant cytoplasm and lacuna formation (chondroid change) |
Collagen arrangement Polarized One field of view, 100× |
Collagen arranged in tightly cohesive well demarcated bundles with a smooth dense bright homogenous polarization pattern with normal crimping | Diminished fibre polarization; separation of individual fibre bundles but with maintenance of overall bundle architecture Non homogeneous polarization | Bundle changes: separation and loss of demarcation of fibre bundles, giving rise to expansion of the tissue overall and clear loss of normal polarization pattern | Marked separation of fibre bundles with complete loss of architecture |
Cellularity One field of view, 100× |
Mainly discrete cells | Hyper cellulara, in runs and/or increased cell numbers | Areas of hypoa as well as hypera cellularity | Area of assessment is mostly a-cellular |
Vascularity ≤ 10 fields of view, 400× |
Inconspicuous blood vessels coursing between bundles | Occasional cluster of vessel, <2 per 10 high power fields | 2–3 clusters of capillaries power 10 high power field | Areas with greater than 3 clusters per 10 high power fields, and/or areas of pathological a-vascularity |
Ground substance One field of view, 100× |
Not stainable ground substance | Stainable mucin between bundles but bundles still discrete | Stainable mucin within bundles with loss of clear demarcation of bundles | Abundant mucin throughout the section with inconspicuous collagen staining |
Hypocellular: < 20 nuclei per field; hypercellular: >30 nuclei per field. Plus 2.5 points for each of: calcification; adipocytes (intratendinous). Total score: a tendon with the most pathology will score 20. A tendon with no observable pathology will score 0.