Figure 1.

Design of Inner Domain Constructs

(A) Subsequent steps of design of the independent ID molecule. Left: structure of gp120_93TH057 core_e in CD4-bound conformation (from PDB code: 4H8W); middle and right: the putative ID1 and ID2 structure. “Layered” architecture of gp120 inner domain is shown with the seven-stranded β sandwich colored violet, layer 1 in yellow, layer 2 in cyan, layer 3 in light orange. The outer domain is shown in gray. Asn-proximal NAG residues of glycosyl group and disulfide bonds of ID are shown as balls and sticks. The -GG(A)- linkers are shown in black. The gp120 surface engaged in binding to the C1-C2 region-specific mAbs is shaded in red.

(B) Sequence of ID constructs of 93TH057 isolate. The -GG- linkers and C₆₅-C₁₁₅ disulfide introduced to ID1 sequence are shown in red.

See also Figure S1.