Figure 6.

Functional Properties of ID2

(A) ID2-competition of Fc-mediated effector functions of anti-Cluster A mAbs. The activities of Cluster A mAbs (at concentrations corresponding to IC₇₅) in (RF)-ADCC assay were measured in the presence of increasing concentrations of ID2 protein using gp120-coated EGFP-CEM-NKr-CCR5-SNAP cells as targets and PBMCs as effector cells.

(B) ADCC competition of Abs in sera from HIV-infected individuals. CEM.NKr cells infected with NL4.3 ADA.GFP Nef⁻Vpu⁻ virus were used at 48 hr post-infection for surface staining of Abs bound (left) and an RF⁻ADCC assay (right) using sera from HIV-1-infected individuals pre-incubated in absence or presence of 10 μg/μl ΔV1V2V3V5 D368R or 2.66 μg/μl ID2 for 30 min at room temperature. Data shown are representative of at least three different experiments. Statistical significance was tested using paired one-way ANOVA (^∗∗p < 0.01, ^∗∗∗∗p < 0.0001).