Table 1.

Binding Kinetics of mAb A32, N5-i5, N60-i3, JR4, and 2.2c to the FLSC, gp120, and ID1 as Measured by Surface Plasmon Resonance

	gp120BaL	FLSC	Fold Difference	ID1	Fold Differencea	Fold Differenceb
mAb A32
KD (M) × 10−9	3.2 ± 0.07	0.23 ± 0.01	13.9	9.30 ± 1.10	2.9	40.4
ka (1/M s) × 105	1.39 ± 0.02	4.43 ± 0.01	3.2	0.85 ± 0.04	1.6	5.2
kd (1/s) ×10−5	43.7 ± 0.99	10.2 ± 0.09	4.3	78.50 ± 5.20	1.8	7.7
mAb N5-i5
KD (M) × 10−9	5.03 ± 0.03	0.29 ± 0.14	17.3	1.10 ± 0.16	4.6	3.8
ka (1/M s) × 105	0.72 ± 0.01	8.25 ± 2.50	11.5	4.50 ± 1.00	6.3	1.8
kd (1/s) ×10−5	36.0 ± 0.28	23.8 ± 13.80	1.5	48.30 ± 4.30	1.3	2.0
mAb N60-i3
KD (M) × 10−9	2.03 ± 0.07	0.25 ± 0.01	8.0	3.50 ± 0.13	1.7	13.8
ka (1/M s) × 105	2.53 ± 0.06	12.4 ± 0.00	4.9	4.30 ± 0.25	1.7	2.9
kd (1/s) ×10−5	51.2 ± 0.50	31.4 ± 0.71	1.6	148 ± 2.70	2.9	4.7
mAb JR4
KD (M) × 10−9	2.34 ± 0.16	0.18 ± 0.01	13.0	6.00 ± 0.33	2.6	33.3
ka (1/M s) × 105	2.62 ± 0.09	6.43 ± 0.06	2.5	1.60 ± 0.10	1.6	4
kd (1/s) ×10−5	61.1 ± 1.80	11.6 ± 0.28	5.3	94.40 ± 0.60	1.5	8.1
mAb 2.2c
KD (M) × 10−9	12.00 ± 0.07	0.50 ± 0.06	24.0	3.00 ± 0.30	4	6.0
ka (1/M s) × 105	0.73 ± 0.01	11.5 ± 1.40	15.7	2.70 ± 0.16	3.7	4.3
kd (1/s) ×10−5	87.5 ± 1.10	55.6 ± 14.40	1.6	80.30 ± 2.40	1.1	1.4

^aFold difference relative to gp120.

^bFold difference relative to FLSC.