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. Author manuscript; available in PMC: 2017 May 3.
Published in final edited form as: Circulation. 2016 Apr 4;133(18):1783–1794. doi: 10.1161/CIRCULATIONAHA.115.020617

Figure 5.

Figure 5

HMGA1 and Slug are necessary for EndMT gene expression changes induced by loss of BMPR2. Commercially available PAECs were transfected using non-targeting siRNA (Con) or siRNA targeting BMPR2 (B2), HMGA1 (H), BMPR2 and HMGA1 (B2+H), Slug (S), or Slug and BMPR2 (B2+S). mRNA normalized to β-actin was assessed by qPCR 72h after transfection. (A) Gene expression levels of BMPR2, HMGA1, Slug and αSMA following siRNA for BMPR2 ±HMGA1. (B) Representative immunoblot and densitometric analysis of BMPR2, HMGA1, Snail/Slug and αSMA protein expression normalized to GAPDH 72h following siRNA for BMPR2±HMGA1. (C) Gene expression levels of BMPR2, Slug and αSMA following siRNA for BMPR2±Slug. Boxplots indicate minimum, maximum and median for n=9 (A and C). Scatterplots indicate mean±SEM for n=3 (in B). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 by one-way ANOVA relative to Con. #P<0.05, ##P<0.01, ###P<0.001, ####P<0.0001 by one-way ANOVA relative to siRNA (B2) treatment. In Panel B, P<0.05, by one-way ANOVA with selected post-hoc comparisons of B2 vs. Con siRNA (§) and B2+H vs. B2 siRNA (+).