Table 3.
Sequence of bovine-specific PCR primers and TaqMan probes to be used for determination of expression of mRNA of AMPKα, MHC-I, MHC-IIA, MHC-IIX, β1AR, β2AR, β3AR, CEBPβ, GPR43, GPR41, Glut4, PPARγ, SCD, and RPS9
| Primer | Sequence (5′ to 3′) |
|---|---|
| AMPkα (accession # NM_001109802) | |
| Forward | ACCATTCTTGGTTGCTGAAACTC |
| Reverse | CACCTTGGTGTTTGGATTTCTG |
| TaqMan probe | 6FAM-CAGGGCGCGCCATACCCTTG-TAMRA |
| MHC-I (accession no. AB059400) | |
| Forward | CCCACTTCTCCCTGATCCACTAC |
| Reverse | TTGAGCGGGTCTTTGTTTTTCT |
| TaqMan probe | 6FAM-CCGGCACGGTGGACTACAACATCATAG-TAMRA |
| MHC-IIA (accession no. AB059398) | |
| Forward | GCAATGTGGAAACGATCTCTAAAGC |
| Reverse | GCTGCTGCTCCTCCTCCTG |
| TaqMan probe | 6FAM-TCTGGAGGACCAAGTGAACGAGCTGA-TAMRA |
| MHC-IIX (accession no. AB059399) | |
| Forward | GGCCCACTTCTCCCTCATTC |
| Reverse | CCGACCACCGTCTCATTCA |
| TaqMan probe | 6FAM-CGGGCACTGTGGACTACAACATTACT-TAMRA |
| β1AR (accession no. AF188187) | |
| Forward | GTGGGACCGCTGGGAGTAT |
| Reverse | TGACACACAGGGTCTCAATGC |
| TaqMan probe | 6FAM-CTCCTTCTTCTGCGAGCTCTGGACCTC-TAMRA |
| β2AR (accession no. NM_174231) | |
| Forward | CAGCTCCAGAAGATCGACAAATC |
| Reverse | CTGCTCCACTTGACTGACGTTT |
| TaqMan probe | 6FAM-AGGGCCGCTTCCATGCCC-TAMRA |
| β3AR (accession no. X85961) | |
| Forward | AGGCAACCTGCTGGTAATCG |
| Reverse | GTCACGAACACGTTGGTCATG |
| TaqMan probe | 6FAM-CCCGGACGCCGAGACTCCAG-TAMRA |
| CEBPβ (accession no. NM_176788) | |
| Forward | CCAGAAGAAGGTGGAGCAACTG |
| Reverse | TCGGGCAGCGTCTTGAAC |
| TaqMan probe | 6FAM-CGCGAGGTCAGCACCCTGC-TAMRA |
| GPR43 (accession no. FJ562212) | |
| Forward | GGCTTTCCCCGTGCAGTA |
| Reverse | ATCAGAGCAGCCATCACTCCAT |
| TaqMan probe | 6FAM-AAGCTGTCCCGCCGGCCC-TAMRA |
| GPR41 (accession no. FJ562213) | |
| Forward | TGCTCCTCAGCACCCTGAA |
| Reverse | TTGGAACCCAGATGATGAGAAA |
| TaqMan probe | 6FAM-TCCTGCGTCGACCCCCTTGTCTAC-TAMRA |
| Glut4 (accession no. D63150) | |
| Forward | CCTCGGCAGCGAGTCACT |
| Reverse | AAACTGCAGGGAGCCAAGAA |
| TaqMan probe | 6FAM-CCTTGGTCCTTGGCGTATTCTCCGC-TAMRA |
| PPARγ (accession no. NM_181024) | |
| Forward | ATCTGCTGCAAGCCTTGGA |
| Reverse | TGGAGCAGCTTGGCAAAGA |
| TaqMan probe | 6FAM-CTGAACCACCCCGAGTCCTCCCAG-TAMRA |
| SCD (accession no. AB075020) | |
| Forward | TGCCCACCACAAGTTTTCAG |
| Reverse | GCCAACCCACGTGAGAGAAG |
| TaqMan probe | 6FAM-CCGACCCCCACAATTCCCG-TAMRA |
| RPS9 (accession no. DT860044) | |
| Forward | GAGCTGGGTTTGTCGCAAAA |
| Reverse | GGTCGAGGCGGGACTTCT |
| TaqMan probe | 6FAM-ATGTGACCCCGCGGAGACCCTTC-TAMRA |
AMPKα AMP-activated protein kinase alpha, MHC-I myosin heavy chain-I, MHC-IIA myosin heavy chain-IIA, MHC-IIX myosin heavy chain-IIX, β1AR beta 1 adrenergic receptor, β2AR beta 2 adrenergic receptor, β3AR beta 3 adrenergic receptor, CEBPβ C-enhancer binding protein beta, GPR43 G-protein coupled receptor 43, GPR41 G-protein coupled receptor 41, Glut4 glucose transporter type 4, PPARγ peroxisome proliferator-activated receptor gamma, SCD stearoyl-CoA desaturase and RPS9 ribosomal protein S9