Fig. 2.

Phagocytosis is associated with the production and the release of EDB fibronectin. a The presence of bacteria does not significantly affect the production of EDB at the mRNA level. Cells were obtained from six healthy subjects and either left untreated or subjected to the addition of bacteria and left at 4 °C for 45 min. EDB was measured by qPCR corrected to the housekeeping gene HPRT. b EDB-containing fibronectin in the cell lysates and in the media was measured by ELISA and corrected to the protein content of the cells measured by the BCA method. N as in A. c Phagocytosis results in an increase in EDB mRNA. Cells were treated with Staphylococcus aureus and left at 37 °C for 10 min and compared to cells treated with S. aureus, but left at 4 °C. N as in A. d Phagocytosis is associated with an increase in EDB-containing fibronectin in the cell lysate or released in the media. e PMA, which induces degranulation, results in increased EDB fibronectin in the media as measured by ELISA. N = 3. f Fibronectin partially colocalizes with lactoferrin found in specific granules. PMNs were isolated, fixed, permeabilzed, and stained for fibronectin in red, lactoferrin in green, and DAPI in blue to visualize the nuclei. Bars represent 2.5 μm