Fig. 6.
Mechanistic studies on the effect of EDB fibronectin and the interaction with other receptors. a Role of Fcγ receptor: Human PMNs were exposed to bacteria opsonized with plasma or with IgG. EDB fibronectin resulted in similar enhancement of phagocytosis. N = 5. b Role of αMβ2: Inhibiting β2 (using an inhibitory antibody) decreases phagocytosis despite the presence of EDB fibronectin. Human PMNs were subjected to phagocytosing plasma-opsonized S. aureus at 37 °C for 10 min. Prior to adding the bacteria, cells were treated with the antibodies (4 μg/ml), N = 7. c Deletion of β1 integrin is successful in Mx-cKO-β1 and is associated with an increase in β2 and β3 expression. Expression was determined by flow cytometry. N = 4 pools of blood from two to three mice/pooled group. d Deletion of β1 integrin results in enhanced phagocytosis that is further increased by EDB fibronectin administration. N = 4 pools of blood from two to three mice/pooled group. e EDB fibronectin release in the media is enhanced in Mx-cKO-β1 cells during phagocytosis. N as in D