Fig. 7.

Studies on the effect of EDB fibronectin on actin polymerization and intracellular signaling. a Actin polymerization was evaluated by phalloidin staining in the presence of opsonized bacteria and pFN or EDB-containing fibronectin for 10 min and mean fluorescence intensity measured. N = 8. b pERK/ERK ratio is increased in PMNs treated with pFN and further enhanced with EDB fibronectin administration 10 min prior to cell lysis. N = 6. c An apparent trend to an increase in pAKT/AKT by Western blotting fails to reach significance in nine replicates/treatment. Human PMNs were prepared as for pERK/ERK ratio. GAPDH was run on a separate gel using the same amount of lysate for technical reasons