Fig. 2.

CSF-1R blockade inhibits tumor growth of tumors with high MDSC infiltration by depletion of CD11b⁺ Gr1^int MDSC subsets. (a) Mice were injected with B16-IDO tumor cells and treated with PLX647 or control chow. Frequencies of Gr^high and Gr^int cell subsets of total CD11b⁺ cells in the spleen, LN, TDLN, and tumor after 2 week treatment with PLX647 or control chow, and representative flow plots of CD11b⁺ Gr1^high and CD11b⁺ Gr1^int cell populations of CD45⁺ cell gated TILs after treatment with PLX647 or control chow. (b) Survival and tumor growth in mice injected with B16-IDO or B16-WT tumor cells and treated with PLX647 or control chow. (c) Tumor weights. (d) Mean tumor growth and overall survival of B16-IDO tumor-bearing mice treated with PLX647, PLX5622 or control chow. Data shown are mean ± SEM of 3 independent experiments.