Fig. 2.

In vitro drug combination studies. Growth inhibition assays performed on BMST (left panel) and BMST-Ras (right panel) cell lines using Alamar Blue after 72 h incubation with doxorubicin (A), paclitaxel (B) and vinblastine (C) alone (black — solid line) or in combination with propranolol at 1 μM (black — broken line), 10 μM (green — solid line) and 50 μM (red — solid line). Points, % of cell proliferation as compared to untreated control cells, means of four individual experiments; bars, 95% confidence interval; log scale for x axis. Statistical analysis was performed by comparing the cytotoxic effect of chemotherapy alone and in combination with propranolol using Student's t test (*, p < 0.05; **, p < 0.01; ***, p < 0.001).