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. 2016 Feb 27;6:139–148. doi: 10.1016/j.ebiom.2016.02.041

Fig. 5.

Fig. 5

Effects of irisin on AMPKα and SREBP2 in primary hepatocytes.

Primary hepatocytes were isolated from either lean or DIO mice and treated with irisin (10 nM) in the presence or absence OA (125 μM). a. Time-dependent effect of irisin on AMPKα phosphorylation. b. SREBP2 content of cytosolic and nuclear fractions of hepatocytes from lean mice analyzed via Western blot. c. Immunofluorescent staining of hepatocytes from lean mice. d. SREBP2 content of cytosolic and nuclear fractions of hepatocytes from DIO mice analyzed via Western blot. e. Immunofluorescent staining of hepatocytes from DIO mice. ***P < 0.001 vs blank control; ###P < 0.001 vs OA. Shown is the representative of at least 3 experiments.