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. 2016 Mar 22;26(5):529–542. doi: 10.1038/cr.2016.33

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Copyright © 2016 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

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Selective markers for the functional switch of Pc from a transcription repressor to an activator. (A) Heat maps of ChIP signals at Pc⁺H3K27me3⁺H4K20me1⁻ and Pc⁺H3K27me3⁺H4K20me1⁺ genes. (B) The Broad/Forkhead motif identified in Pc⁺H3K27me3⁺H4K20me1⁺ genes. (C) RT-qPCR analysis of mRNA levels of Br, Ry, Spn100A and Sens in Act5c-Gal4 or Act5c-Gal4/uas-dsRNA Br wing discs. (D) S2 cells were transfected with combinations of DNA constructs as indicated. After 48 h of transfection, lysates from transfected S2 cells were immunoprecipitated with anti-V5 antibody or anti-Myc antibody. Western blots were performed to analyze the presence of Flag-, V5- or Myc-tagged proteins. (E) Gel filtration chromatography on a Superose 6 column and western blot of Cl-8 nuclear extract. Molecular weight standards are shown on top (in kDa). Means ± SD; n = 3. ^*P < 0.05, ^**P < 0.01. Unpaired, two-tailed Student's t-test. See also Supplementary information, Figure S5.