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. 2016 Apr 8;26(5):543–555. doi: 10.1038/cr.2016.45

Figure 5.

Figure 5

Mutagenesis analysis of PXY-TDIF complex. (A) Effects of TDIF mutations on interaction with PXYLRR. GST-TDIF and its mutants were individually incubated with the purified PXYLRR protein and then flowed through GS4B resin bound by GST-TDIF or its mutants. After extensive washing, the GS4B resin-bound proteins were visualized by Coomassie blue staining following SDS-PAGE. 13R had an additional arginine added at the C terminus of TDIF. (B) Effects of PXY mutations on interaction with GST-TDIF. The assay was performed as described in Figure 1A. For (A and B), blue and green arrows indicate PXYLRR and GST-TDIF, respectively. (C and D) The vascular phenotype of WT and pxy-5 mutant. (E) The PXY genomic sequence completely rescued the phenotype of pxy-5. (F-K) Complementation results of the mutated PXY genes. (C-F) Upper lane: resin-embedded transverse sections of stem vascular bundles of indicated plants. Scale bars: 10 μm. Lower lane: corresponding hand-cut transverse sections of stem vascular bundles. Scale bars: 20 μm. Ph: phloem; C: cambium; Xy: xylem. Red brackets enclose layer structures of cambium, arrows indicate discontinuous cambial cells.