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Validation experiments including the optimum point with the corresponding predicted and actual phytase activities.
| Run no. | Temperature (°C) | pH | Phytate concentration (mM) | Predicted phytase activity (U/mL) | Actual phytase activity (U/mL) |
|---|---|---|---|---|---|
| 1 | 50.0 | 6.5 | 1.50 | 3.586 | 3.337 |
| 2 | 60.0 | 7.0 | 1.00 | 3.203 | 3.631 |
| 3 | 45.0 | 6.0 | 2.00 | 2.454 | 2.522 |
| 4a | 56.5 | 7.3 | 2.05 | 4.661 | 4.627 |
Optimum point.
