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. 2016 Apr 5;6(3):198–204. doi: 10.1016/j.apsb.2016.03.005

Figure 4.

Fig. 4

E17110 reduces ox-LDL-induced lipid accumulation in RAW264.7 macrophages. RAW264.7 macrophages were preincubated with (A) PBS for vehicle and (B)–(E) ox-LDL (60 μg/mL) for samples. After 24 h, these cells were separately treated with (B) DMSO, (C)–(E) E17110 (1, 3 and 10 μmol/L) for 18 h. The cells were fixed with 4% paraformaldehyde and stained with 0.5% oil red O to detect lipid accumulation. Representative images of the five study group samples are shown (×400 magnification). Similar results were obtained in three independent experiments. (F) PBS, ox-LDL (60 μg/mL) and E17110 (1, 3 and 10 μmol/L) were added to the cultures throughout the experiment. After oil red O staining, bound dye was solubilized and quantified spectrophotometrically at 510 nm.