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. 2015 Dec 15;44(8):3586–3594. doi: 10.1093/nar/gkv1481

Figure 2.

Figure 2.

H3K9 methyltransferase activity and global H3K9me3 levels. (A) Graph of H3K9-specific methyltransferase activity of native protein isolated from the nuclear fraction of MCF7 cells after transfection with FLAG-KLLN or vector alone, and with and without siRNA-based knockdown of KLLN. H3K9 specific methyltransferase activity is increased with overexpression of KLLN in MCF7 cells (P = 0.017). Knockdown of KLLN decreased methyltransferase activity (P = 0.012). (B) Similar graph of H3K9 methyltransferase activity in MCF10A cells. KLLN overexpression increased methyltransferase activity (P = 0.023) whereas knockdown of KLLN decreased the same. (C) Immunoblot analysis of native protein isolated from the nuclear fraction of MCF7 cells for global H3K9 trimethylation levels after transfection with FLAG-KLLN or vector alone, and with and without siRNA-based knockdown of KLLN. Overexpression of KLLN increased global H3K9me3 levels whereas knockdown of KLLN decreased these levels. (D) Similar immunoblot analysis of global H3K9me3 levels in MCF10A cells. Increased global H3K9me3 after overexpression of KLLN while knockdown of KLLN decreased the same.