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. 2015 Dec 23;44(8):3610–3617. doi: 10.1093/nar/gkv1496

Figure 1.

Figure 1.

Inhibition of class I HDAC activity represses adrenergic stimulated Ncx1 promoter activation. Isolated adult cardiomyocytes were infected with adenoviruses containing 1831Ncx1 promoter luciferase reporter construct (multiplicity of infection (MOI) 1.5) and treated with either 1 μM isoproterenol (Iso) or 10 μM phenylephrine (PE) following pretreatment with either broad HDAC inhibitor, (A) SAHA Class I selective HDAC inhibitor or (B) MS275 (1 μM). (C) Isolated adult cardiomyocytes were infected with adenoviruses containing 1831Ncx1 promoter luciferase reporter construct (MOI 1.5) and treated with either 1 μM isoproterenol (Iso) or 10 μM phenylephrine (PE) following pretreatment with Class IIa HDAC inhibitor, dPAHA (10 μM). Cells for (A, B and C) were lysed in reporter buffer 24 h after infection and luciferase activity was determined relative to GFP levels. MS275 significantly inhibited Ncx1 promoter upregulation. PAHA had no effect on Ncx1 promoter activity. *P < 0.05 respective to control, #P < 0.05 respective to ± PE or ISO treatment (Student's t-test; n = 4).