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. 2015 Dec 23;44(8):3618–3628. doi: 10.1093/nar/gkv1502

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Frequent co-localization between Pol III gene loci present on same chromosomes. (A) FISH visualization of the Pol III gene loci located on the same and different chromosomes. The c10H11 Pol III gene locus on the chromosome 3 was co-visualized with either the c417 Pol III gene locus (chr3) or the c354 Pol III gene locus (chr2). Representative FISH images are shown on top. Centers of FISH foci were defined as positions of the loci. The distance between the two loci was measured in more than 100 cells and assigned to a 0.1 μm bin. (B) Simplified representation of the FISH results shown in panel A. The distance between the two loci was binned into one of the three categories (right). This is to simplify a representation of the FISH data, and statistical analysis was performed based on the original datasets (the Mann–Whitney U test). (C) Positions of the gene loci visualized by FISH experiments. The cosmid clones (yellow) were used to prepare FISH probes. The cosmid names in shade indicate the negative control loci without Pol III genes, and this representation is consistent in other figures. Positions of Pol III genes (tRNA and 5S rRNA) are also shown. (D) Pairs of the indicated gene loci were co-visualized by FISH, and FISH data were summarized as described in panel B. Numbers of Pol III genes present at the gene loci are annotated beneath the cosmid clones. (E and F) The Pol III genes (tRNA^asn and 5S rRNA) were inserted to the c162 non-Pol III gene locus. The c162 control locus with and without the insertion of the Pol III genes was co-visualized with the c10H11 Pol III gene locus (E). FISH data were summarized as described in panel B (F).