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Pif1 stimulates the strand displacement activity of Pol δ. (A) Primer extension assays using the indicated DNA (25 nM) and PCNA-loaded Pol δ (25 nM) performed in the absence and presence of different versions of Pif1 (20 nM). Times (t) are: 5”, 15”, 30”, 60”. (B) Replication assays of a 18 nt gapped pUC19 (10 nM) with PCNA-loaded Pol δDV (15 nM) in the absence or presence of the shorter version of Pif1 (10 or 50 nM) or its ATPase inactive variant (50 nM).
