Skip to main content
. 2016 Apr 7;44(8):3481–3494. doi: 10.1093/nar/gkw190

Table 1. Wild-type and trapped mutant GQ sequences.

Name Sequence Tm °Ca
VEGFA-WT 5′-GGGAGGGTTGGGGTGGG-3′ 61.2 ± 0.1
VEGFA-1 5′-GGGAGGGTTIGGGTGGG-3′ 58.1 ± 0.1
VEGFA-2 5′-GGGAGGGTTGGGITGGG-3′ 57.7 ± 0.1
c-mycPu18 WT 5′-AGGGTGGGGAGGGTGGGG-3′ 79.9 ± 0.2b
c-myc Pu18 55 5′-AGGGTTGGGAGGGTTGGG-3′ 66.2 ± 0.1
5′-AGGGTIGGGAGGGTIGGG-3′ 62.00 ± 0.04
c-myc Pu18 53 5′-AGGGTTGGGAGGGTGGGT-3′ 69.4 ± 0.1
5′-AGGGTIGGGAGGGTGGGI-3′ 65.60 ± 0.04
c-myc Pu18 35 5′-AGGGTGGGTAGGGTTGGG-3′ 73.6 ± 0.1
5′-AGGGTGGGIAGGGTIGGG-3′ 74.40 ± 0.04
c-myc Pu18 33 5′-AGGGTGGGTAGGGTGGGT-3′ 77.3 ± 0.1
5′-AGGGTGGGIAGGGTGGGI-3′ 77.20 ± 0.03
PIM1-WT 5′-GGGCGGGGCGGGGGCGGGG-3′ 68.8 ± 1.6b
PIM1-1 5′-GGGCTGGGCTTGGGCTGGG-3′ 54.5 ± 0.1
5′-GGGCIGGGCIIGGGCIGGG-3′ 50.0 ± 0.1
PIM1-2 5′-GGGCTGGGCTTGGGCGGGT-3′ 56.7 ± 0.2
5′-GGGCIGGGCIIGGGCGGGI-3′ 53.4 ± 0.2
PIM1-3 5′-GGGCTGGGCTGGGTCTGGG-3′ 52.5 ± 0.1
5′-GGGCIGGGCIGGGICIGGG-3′ 49.1 ± 0.1
PIM1-4 5′-GGGCTGGGCTGGGTCGGGT-3′ 55.7 ± 0.2
5′-GGGCIGGGCIGGGICGGGI-3′ 50.2 ± 0.2
PIM1-5 5′-GGGCTGGGCGGGTTCTGGG-3′ 55.9 ± 0.2
5′-GGGCIGGGCGGGIICIGGG-3′ 49.4 ± 0.4
PIM1-6 5′-GGGCTGGGCGGGTTCGGGT-3′ 56.4 ± 0.2
5′-GGGCIGGGCGGGIICGGGI-3′ 52.9 ± 0.2
PIM1-7 5′-GGGCGGGTCTTGGGCTGGG-3′ 52.4 ± 0.1
5′-GGGCGGGICIIGGGCIGGG-3′ 53.6 ± 0.1
PIM1-8 5′-GGGCGGGTCTTGGGCGGGT-3′ 61.2 ± 0.1
5′-GGGCGGGICIIGGGCGGGI-3′ 61.8 ± 0.1
PIM1-9 5′-GGGCGGGTCTGGGTCTGGG-3′ 51.4 ± 0.2
5′-GGGCGGGICIGGGICIGGG-3′ 51.1 ± 0.1
PIM1-10 5′-GGGCGGGTCTGGGTCGGGT-3′ 54.7 ± 0.2
5′-GGGCGGGICIGGGICGGGI-3′ 55.3 ± 0.1
PIM1-11 5′-GGGCGGGTCGGGTTCTGGG-3′ 51.8 ± 0.2
5′-GGGCGGGICGGGIICIGGG-3′ 48.4 ± 0.3
PIM1-12 5′-GGGCGGGTCGGGTTCGGGT-3′ 55.8 ± 0.5
5′-GGGCGGGICGGGIICGGGI-3′ 53.3 ± 0.2

aDerived from UV-Vis analysis.

bAverage Tm from global fitting of dT and dI trapped mutant data sets.