Table 1. Wild-type and trapped mutant GQ sequences.
Name | Sequence | Tm °Ca |
---|---|---|
VEGFA-WT | 5′-GGGAGGGTTGGGGTGGG-3′ | 61.2 ± 0.1 |
VEGFA-1 | 5′-GGGAGGGTTIGGGTGGG-3′ | 58.1 ± 0.1 |
VEGFA-2 | 5′-GGGAGGGTTGGGITGGG-3′ | 57.7 ± 0.1 |
c-mycPu18 WT | 5′-AGGGTGGGGAGGGTGGGG-3′ | 79.9 ± 0.2b |
c-myc Pu18 55 | 5′-AGGGTTGGGAGGGTTGGG-3′ | 66.2 ± 0.1 |
5′-AGGGTIGGGAGGGTIGGG-3′ | 62.00 ± 0.04 | |
c-myc Pu18 53 | 5′-AGGGTTGGGAGGGTGGGT-3′ | 69.4 ± 0.1 |
5′-AGGGTIGGGAGGGTGGGI-3′ | 65.60 ± 0.04 | |
c-myc Pu18 35 | 5′-AGGGTGGGTAGGGTTGGG-3′ | 73.6 ± 0.1 |
5′-AGGGTGGGIAGGGTIGGG-3′ | 74.40 ± 0.04 | |
c-myc Pu18 33 | 5′-AGGGTGGGTAGGGTGGGT-3′ | 77.3 ± 0.1 |
5′-AGGGTGGGIAGGGTGGGI-3′ | 77.20 ± 0.03 | |
PIM1-WT | 5′-GGGCGGGGCGGGGGCGGGG-3′ | 68.8 ± 1.6b |
PIM1-1 | 5′-GGGCTGGGCTTGGGCTGGG-3′ | 54.5 ± 0.1 |
5′-GGGCIGGGCIIGGGCIGGG-3′ | 50.0 ± 0.1 | |
PIM1-2 | 5′-GGGCTGGGCTTGGGCGGGT-3′ | 56.7 ± 0.2 |
5′-GGGCIGGGCIIGGGCGGGI-3′ | 53.4 ± 0.2 | |
PIM1-3 | 5′-GGGCTGGGCTGGGTCTGGG-3′ | 52.5 ± 0.1 |
5′-GGGCIGGGCIGGGICIGGG-3′ | 49.1 ± 0.1 | |
PIM1-4 | 5′-GGGCTGGGCTGGGTCGGGT-3′ | 55.7 ± 0.2 |
5′-GGGCIGGGCIGGGICGGGI-3′ | 50.2 ± 0.2 | |
PIM1-5 | 5′-GGGCTGGGCGGGTTCTGGG-3′ | 55.9 ± 0.2 |
5′-GGGCIGGGCGGGIICIGGG-3′ | 49.4 ± 0.4 | |
PIM1-6 | 5′-GGGCTGGGCGGGTTCGGGT-3′ | 56.4 ± 0.2 |
5′-GGGCIGGGCGGGIICGGGI-3′ | 52.9 ± 0.2 | |
PIM1-7 | 5′-GGGCGGGTCTTGGGCTGGG-3′ | 52.4 ± 0.1 |
5′-GGGCGGGICIIGGGCIGGG-3′ | 53.6 ± 0.1 | |
PIM1-8 | 5′-GGGCGGGTCTTGGGCGGGT-3′ | 61.2 ± 0.1 |
5′-GGGCGGGICIIGGGCGGGI-3′ | 61.8 ± 0.1 | |
PIM1-9 | 5′-GGGCGGGTCTGGGTCTGGG-3′ | 51.4 ± 0.2 |
5′-GGGCGGGICIGGGICIGGG-3′ | 51.1 ± 0.1 | |
PIM1-10 | 5′-GGGCGGGTCTGGGTCGGGT-3′ | 54.7 ± 0.2 |
5′-GGGCGGGICIGGGICGGGI-3′ | 55.3 ± 0.1 | |
PIM1-11 | 5′-GGGCGGGTCGGGTTCTGGG-3′ | 51.8 ± 0.2 |
5′-GGGCGGGICGGGIICIGGG-3′ | 48.4 ± 0.3 | |
PIM1-12 | 5′-GGGCGGGTCGGGTTCGGGT-3′ | 55.8 ± 0.5 |
5′-GGGCGGGICGGGIICGGGI-3′ | 53.3 ± 0.2 |
aDerived from UV-Vis analysis.
bAverage Tm from global fitting of dT and dI trapped mutant data sets.