Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Apr 1;44(8):3801–3810. doi: 10.1093/nar/gkw214

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 2. — CRISPR/dCas9 inhibition of replication initiation is highly specific to the origin of replication region. (A) Genetic map of the oriC region. The 245 bp oriC sequence is marked as well as two adjacent gene-coding regions. Each number corresponds to a different gRNA 20nt target. Targets complementary to origin of replication are shown in red, targets binding outside of origin region are marked in green. Double line represents the dsDNA; sgRNA targets binding the leading strand are represented to bind the upper strand; gRNA targets complementary to the lagging strand are represented to bind the bottom strand. DnaA boxes from left to right: R1, R2, R4. (B and C) CRPSR/dCas9 inhibits cell growth only when targeted to origin of replication. Serial 10-fold dilutions of bacterial cultures were plated on media without the addition (B) or with addition (C) of 200 ng/ml of aTc. Numbers corresponds to gRNA targets shown on (A).