. Author manuscript; available in PMC: 2016 May 5.

Published in final edited form as: Mol Pharmacol. 2012 Nov 27;83(2):439–453. doi: 10.1124/mol.112.082321

TABLE 3.

Kinetic parameters of pyrimidine transport in bloodstream forms of wild-type and 5-FURes T. b. brucei

[³H]-Permeant	Strain	K_m	V_max	V_max/K_m
[³H]-Permeant	Strain	μM	pmol (10⁷ cells)⁻¹s⁻¹	V_max/K_m
Uracil	s427WT	1.5 ± 0.3	0.27 ± 0.05	0.18
	5-FURes	0.66 ± 0.15	0.16 ± 0.02	0.25
5-FU	s427WT	2.5 ± 0.01	0.27 ± 0.02	0.11
	5-FURes	2.3 ± 0.4	0.20 ± 0.02	0.088
Uridine	s427WT	9500 ± 2700	16 ± 4	0.0017
2′-Deoxyuridine	s427WT	810 ± 310	1.3 ± 0.7	0.0017
Thymidine	s427WT	1240 ± 310	0.067 ± 0.008	0.0001