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. 2016 May 5;6:25370. doi: 10.1038/srep25370

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(A) Turbidity was monitored with a Loopamp real-time turbidimeter at 400 nm every 6 s. (B) A visual colour change detection method was compared. 1 μl of calcein (a fluorescent detection reagent) was added to 25 μl of the LAMP reaction mixture before the LAMP reaction. Lines and tubes: 1, C. sativus 01; 2, C. sativus 11; 3, C. sativus 12; 4, C. sativus 13; 5, C. officinalis 01; 6, C. tinctorius 01; 7, C. longa 01; 8, D. carota 01; 9, N. nucifera 01; 10, Z. mays 01; and 11, Neg (negative control, ddH₂O).