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. 2016 May 5;6:24990. doi: 10.1038/srep24990

Figure 6. Inhibition of nitric oxide synthesis balancing the mRNA transcript levels of genes regulating inflammation, and oxidative stress condition.

Figure 6

Inhibition of NO synthesis by treatment with a low dose, 0.01 mg/mL, of L-NAME for 9 weeks (LN9w) in drinking water was performed in WT (open bar) and KO (closed bar) mice starting from 12 weeks of age. Untreated mice were used as control (cont). (A) L-NAME lowers the total nitrite and nitrate level in the serum of L-NAME treated WT and KO mice compared with untreated control. (B) Effect of L-NAME in the expression of αSma, Tnf-α, and Ccl-2 mRNA in the liver. (C) Effect of L-NAME in the expression of oxidative stress regulated genes in WT and KO mouse livers. Note the undetectable level of the Mpo transcript gene (depict as 0) together with the very low expression of Ho-1 in both WT and KO mice under L-NAME treatment. Data represent the mean ± SD; n = 5–10 for each group; *p < 0.05; **p < 0.01; ***p < 0.001; a, p < 0.05 for comparisons of the WT and KO control with the L-NAME treatment using one sample t-test with a hypothetical mean value of 0.