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. 2016 May 5;6:25502. doi: 10.1038/srep25502

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(a) Untreated and TNFα-treated (10 ng/ml, 16 hours) EC were lysed and protein levels of RhoA/B/C were detected on Western blot. Actin was detected to control for equal loading. (b) EC were transfected with the RhoA/B/C FRET sensors as indicated. YFP/CFP ratios (±SEM) were compared for untreated RhoA (n = 47), RhoB (n = 65), RhoC (n = 30) and TNFα-treated (10 ng/ml, 16 hours) RhoA (n = 41), RhoB (n = 70), RhoC (n = 28) sensor-expressing EC, ns = P > 0.05, ****P<0.0001, Student’s t test. (c) Normalized mean YFP/CFP ratios (±SEM) of EC that were transfected with the RhoA (n = 11), RhoB (n = 13) or RhoC (n = 12) FRET sensor and stimulated with TNFα at t = 110 sec.