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. 2016 May 5;6:25502. doi: 10.1038/srep25502

Figure 8. The cyclic AMP-analogue 007 induces RhoA/B/C inactivation and accumulation of RhoC at cell-cell contacts.

Figure 8

(a) Normalized mean YFP/CFP ratios (±SEM) of EC that were transfected with the RhoA (n = 11), RhoB (n = 14) or RhoC (n = 10) FRET sensors and stimulated with 007 (1 mM) at t = 110 sec. (b) Untreated and 007-treated (1 mM, 30 minutes) EC were stained for RhoC and VE-cadherin. Box selections in control cells show colocalization of RhoC and VE-cadherin and less stabilized VE-cadherin. Boxes in 007-treated cells show co-localization of RhoC and VE-cadherin, accumulation of RhoC at cell-cell contacts and more stabilized VE-cadherin. Scale bar = 14 μm. (c) Profile plots show gray values of RhoC (green line) and VE-cadherin (blue line) of untreated and 007-treated EC according to the line present in the corresponding images in (b).