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. 2016 May 5;6:25447. doi: 10.1038/srep25447

Figure 2. IL-17B levels are increased in peripheral blood of gastric cancer (GC) patients.

Figure 2

(a) Real-time RT-PCR analyses of IL-17B mRNA levels in gastric tumors and paired adjacent mucosa (n = 60; ns, no significant). (b) The correlation of IL-17RB and IL-17B mRNA expression in gastric cancer tissues (r = 0.01242, p = 0.9385). (c) ELISA analyses the levels of IL-17B in the serum of gastric cancer patients and normal volunteers (n = 60 vs 40; ***p = 0.0003). (d) RT-PCR analyses of IL-17RB and IL-17B mRNA expression in SGC-7901, MKN-45, BGC-823, MGC-803, and HGC-27 human GC cells. (e) Western blot analyses of IL-17RB protein expression in SGC-7901, MKN-45, BGC-823, MGC-803, and HGC-27 human GC cells, the blots were cropped from the same gel. (f) Representative images of cell colonies in control and IL-17RB-shRNA-transfected MGC-803 cells. (g) The migration of IL-17RB knockdown MGC-803 cells and control cells for 24 h and 48 h was detected by using a cell scratch assay (n = 3).