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. 2016 May 5;6:25447. doi: 10.1038/srep25447

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Copyright © 2016, Macmillan Publishers Limited

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Real-time RT-PCR (a) and Western blots (b) analyses of IL-17RB in MGC-803 cells treated with corresponding concentrations of rIL-17B for 48 h. Real-time RT-PCR analyses of LGR5 (c), Oct4 (d), Sall4 (e), and Sox2 (f) mRNA levels in MGC-803 cells treated with corresponding concentrations of rIL-17B for 48 h. (g) Western blots detecting the protein expression of IL-17RB, E-cadherin, N-cadherin, Nanog, Sox2, and Oct4 in IL-17RB knockdown cells and control MGC-803 cells treated with corresponding concentrations of rIL-17B for 48 h, the blots were cropped from different gels but all the gels had been run under the same experimental conditions. (h) MGC-803 cells treated with corresponding concentrations of rIL-17B for 48 h, then induced with adipogenic differentiation medium. Representative images show accumulation of lipid droplets after undergoing adipogenic differentiation.