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. 2016 May 5;6:25507. doi: 10.1038/srep25507

Figure 4. Cd36 expressing neurons coexpress canonical OSNs transcripts.

Figure 4

(a) Two-color fluorescent in situ hybridization (FISH) with a Cd36 riboprobe shown in green (white arrowheads) and the other transcripts shown in red (empty arrowheads); arrows indicate coexpression (Omp and Gnal). While a high frequency of coexpression was observed for the canonical OSNs transcripts, no Cd36 coexpression was observed for two ORs (Olfr1507 and P2/Olfr17) or members of the Taar7 subfamily (identified with a Taar7b probe) – please refer to Table 1 for details. In the bottom panel, one-color FISH with a Cd36 riboprobe (green) combined with immunostaining for the GFP protein (red) was performed to identify the P2-IRES-tauGFP expressing neurons. Supporting cells (Cyp2a4 labeled apical cells; delimited with dashed lines) show weaker labeling for Cd36 when compared to OSNs. (b) Confocal acquisitions with orthogonal projections depicting Cd36+ OSNs coexpressing Gnal and Omp transcripts. Nuclei were stained with DAPI (blue). Scale bars are shown.