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. 2016 May 5;6:25399. doi: 10.1038/srep25399

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(A) Epididymal white adipose tissue (eWAT) weight. (B) Epididymal adipose tissue sections by hematoxylin and eosin (H&E) staining. Arrows indicate macrophage-like cells. Bar represents 50 μm. (C) Average diameter of adipocytes of eWAT. Adipocyte sizes were quantified from 100 adipocytes from 10 fields. (D) The gene expressions of key transcriptional factors in adipogenesis. (E) The expressions of genes involved in triglyceride synthesis. (F) The adipocytokine gene expressions. (G) The plasma leptin levels. (H) Leptin signaling pathway in hypothalamus. (I) Densitometric quantification of the immunoblot data in (H). The p-STAT3 Tyr705 protein levels were normalized to total STAT3 protein levels. n = 5. All values represent mean ± SEM from three independent experiments. All data except in (I) compared by Student’s t test. Data in (I) were analyzed by two-way ANOVA analysis. *p < 0.05, **p < 0.01.