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. 2016 May 5;6:25594. doi: 10.1038/srep25594

Figure 4. Notch activation decreased the expression of PPM1A.

Figure 4

ATDC5 cells were transfected with siRNA oligos or plasmids for 48 hours before being harvested for western blot and PCR analysis. (A) PPM1A gene expression was measured by Real time RT-PCR in NICD1 expressing and/or SMAD1/5/8 knocking down ATDC5 cells after culture for 48 hours. (B) p-SMAD1/5/8 and PPM1A protein levels were measured by Western blot analysis in ATDC5 cells transfected with NICD1 and/or PPM1A plasmids. (C) Quantization of band density of PPM1A in western blot results. (D) Quantization of band density of p-SMAD1/5/8 in western blot results. PCR data are means ± SD of three independent experiments performed in duplicate and the control expression level was set at 1. (*p < 0.01 compared with vehicle control; #p < 0.05 compared with siSMAD1/5/8 or PPM1A alone cells).