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. 2016 May 5;6:25579. doi: 10.1038/srep25579

Figure 2. Effect of cycloheximide and actinomycin D on curcumin-induced AT1R downregulation.

Figure 2

(A) The effect of cycloheximide (CHX) on curcumin (CUR)-induced AT1R protein down-regulation was examined by Western blot analysis; cropped blots are shown. The AT1R expression level was normalized against β-actin (*P < 0.05 vs. control, #P < 0.05 vs. CHX, n = 7). (B) The effect of actinomycin D (ActD) on CUR-induced AT1R mRNA down-regulation was examined by RT-PCR analysis. The AT1R expression was normalized against β-actin. The values are expressed as the mean ± SEM (*P < 0.05 vs. control, n = 7).