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. 2016 May 5;6:25579. doi: 10.1038/srep25579

Figure 4. Effect of curcumin on the binding ability of SP1 at the AT1R gene promoter in A10 cells.

Figure 4

The binding activity of the AT1R gene promoter (−40 bp to −6 bp), which contains an Sp1 site, was examined in the nuclear protein from unstimulated (lane 2) or curcumin (10−6 M)-stimulated (lane 3) A10 cells by electrophoretic mobility shift assay; DNA-binding protein (arrow) was decreased compared with unstimulated A10 cells. No nuclear extracts or 50 times of unlabeled probe were added to the reaction mixture as controls (lanes 1 and 4, respectively).