Table 5.
Hematological profile in non-infected and infected horses with T. equi and/or B. caballi
| Groups Parameters |
Control Non-infected |
Diagnostic techniques performed on infected horses | Sig. | |||
|---|---|---|---|---|---|---|
| Blood film | cELISA | nPCR | Blood film + ELISA + PCR | |||
| No. of Animals | 12 | 12 | 9 | 9 | 6 | |
| Red blood cell count (×106/μl) | 7.54 ± 0.19a | 6.59 ± 0.23b | 6.74 ± 0.30b | 6.33 ± 0.13b | 6.65 ± 0.31b | ** |
| Haematocrit (%) | 32.79 ± 0.86 | 31.14 ± 0.80 | 31.40 ± 1.52 | 29.01 ± 0.43 | 30.75 ± 2.22 | NS |
| Haemoglobin (g/dl) | 11.91 ± 0.27a | 10.96 ± 0.26ab | 11.20 ± 0.49ab | 10.37 ± 0.12b | 11.03 ± 0.66ab | * |
| Mean corpuscular volume (fl) | 43.53 ± 0.70b | 47.42 ± 0.53a | 46.59 ± 0.77a | 45.90 ± 0.58a | 46.07 ± 1.47 | ** |
| Mean corpuscular haemoglobin (pg) | 15.82 ± 0.18b | 16.71 ± 0.25a | 16.61 ± 0.12a | 16.47 ± 0.29ab | 16.57 ± 0.37a | * |
| MCHC (mg/dl) | 36.40 ± 0.30 | 35.23 ± 0.29 | 35.73 ± 0.40 | 35.86 ± 0.24 | 36.08 ± 0.45 | NS |
| Red blood cell distribution width (fl) | 37.89 ± 1.08 | 40.96 ± 0.66 | 40.84 ± 1.04 | 41.04 ± 0.48 | 40.83 ± 2.35 | NS |
| Red blood cell distribution width (%) | 21.23 ± 0.48 | 20.13 ± 0.69 | 20.77 ± 0.27 | 21.72 ± 0.48 | 21.30 ± 0.83 | NS |
| White blood cell count(×103/μl) | 7.47 ± 0.32 | 7.60 ± 0.13 | 8.00 ± 0.71 | 6.36 ± 0.36 | 7.63 ± 0.62 | NS |
| Lymphocytes(×103/μl) | 1.65 ± 0.11 | 1.54 ± 0.10 | 1.63 ± 0.23 | 1.34 ± 0.14 | 1.95 ± 0.29 | NS |
| Lymphocytes (%) | 22.22 ± 1.36 | 20.32 ± 1.33 | 20.12 ± 1.35 | 22.06 ± 2.08 | 25.50 ± 2.53 | NS |
| Granulocytes (×103/μl) | 5.19 ± 0.28ab | 5.53 ± 0.16a | 5.69 ± 0.45a | 4.34 ± 0.32b | 5.05 ± 0.42ab | * |
| Granulocytes (%) | 69.38 ± 1.54 | 72.68 ± 1.62 | 72.04 ± 1.23 | 68.22 ± 2.55 | 66.80 ± 3.06 | NS |
| Mid cells(×103/μl) | 0.63 ± 0.03 | 0.53 ± 0.03 | 0.68 ± 0.06 | 0.67 ± 0.09 | 0.63 ± 0.06 | NS |
| Mid cells (%) | 8.39 ± 0.36ab | 7.00 ± 0.33b | 7.83 ± 0.36b | 9.72 ± 0.85a | 7.70 ± 0.64b | ** |
| Platelet count(×103/μl) | 220.58 ± 16.98a | 136.17 ± 5.28b | 140.22 ± 10.57b | 139.67 ± 11.97b | 152.67 ± 12.00b | *** |
| Mean platelet volume (fl) | 8.28 ± 0.25a | 7.30 ± 0.21b | 7.32 ± 0.17b | 8.06 ± 0.15ab | 7.77 ± 0.30b | ** |
| Platelet distribution width (fl) | 14.58 ± 0.34a | 12.78 ± 0.49c | 13.21 ± 0.25bc | 14.20 ± 0.29ab | 13.50 ± 0.53abc | ** |
| Plateletcrit (%) | 0.19 ± 0.02a | 0.10 ± 0.01b | 0.10 ± 0.01b | 0.11 ± 0.01b | 0.12 ± 0.01b | *** |
| Large platelet concentration ratio | 32.57 ± 2.45a | 21.03 ± 2.33c | 22.59 ± 1.16bc | 28.42 ± 1.84ab | 25.25 ± 3.28bc | ** |
Hemogram values expressed as mean ± standard error for each technique. Means followed by different superscripts (a, b, c) within the same row are significantly different at (P < 0.05). Two means that are not follow by the same letter (a, b, or c) are significantly different (P < 0.05). Two means followed with the same letter implies that they are not significantly different. Sig. represents statistical significance: *P < 0.05; **P <0.01; ***P <0.001; NS, non-significant